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pcdna3 tlr7 yfp addgene (Addgene inc)

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Addgene inc pcdna3 tlr7 yfp addgene
Pcdna3 Tlr7 Yfp Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+tlr7+yfp/pcDNA3-TLR7-YFP+(Plasmid+%2313022)/pm40886313-804-160-161
Average 92 stars, based on 7 article reviews

pcdna3 tlr7 yfp addgene - by Bioz Stars, 2026-10

92/100 stars

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Article Title: ACE2-containing defensosomes serve as decoys to inhibit SARS-CoV-2 infection.
Article Snippet: A549ACE2 cells were generated using lentiviral transduction of a human ACE2 cDNA-expressing plasmid (backbone: pLV-EF1a-IRES-Puro (Addgene 85132)) as previously described [34]. .. TLR7+ A549 were generated by lentiviral transduction of pcDNA3-TLR7-YFP (a gift from Doug Golenbock (Addgene plasmid # 13022; http://n2t.net/addgene:13022; RRID:Addgene_13022)). ..

Article Title: ACE2-containing defensosomes serve as decoys to inhibit SARS-CoV-2 infection
Article Snippet: A549 ACE2 cells were generated using lentiviral transduction of a human ACE2 cDNA-expressing plasmid (backbone: pLV-EF1a-IRES-Puro (Addgene 85132)) as previously described [ ]. .. TLR7+ A549 were generated by lentiviral transduction of pcDNA3-TLR7-YFP (a gift from Doug Golenbock (Addgene plasmid # 13022; http://n2t.net/addgene:13022 ; RRID:Addgene_13022)). ..

Article Title: ACE2-containing defensosomes serve as decoys to inhibit SARS-CoV-2 infection
Article Snippet: A549 ACE2 cells were generated using lentiviral transduction of a human ACE2 cDNA expressing plasmid (backbone: pLV-EF1a-IRES-Puro (Addgene 85132) as previously described [ ]. .. TLR7+ A549 were generated by lentiviral transduction of pcDNA3-TLR7-YFP (a gift from Doug Golenbock (Addgene plasmid # 13022; http://n2t.net/addgene:13022 ; RRID:Addgene_13022)). ..

Transduction:

Article Title: ACE2-containing defensosomes serve as decoys to inhibit SARS-CoV-2 infection
Article Snippet: A549 ACE2 cells were generated using lentiviral transduction of a human ACE2 cDNA expressing plasmid (backbone: pLV-EF1a-IRES-Puro (Addgene 85132) as previously described [ ]. .. TLR7+ A549 were generated by lentiviral transduction of pcDNA3-TLR7-YFP (a gift from Doug Golenbock (Addgene plasmid # 13022; http://n2t.net/addgene:13022 ; RRID:Addgene_13022)). ..

Plasmid Preparation:

Article Title: Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNF-α in monocytes
Article Snippet: .. To validate that RNA extracted from RA SF specifically ligates to TLR7, NL monocytes and 293T cells were either untransfected or transfected with pCDNA3-TLR7-YFP (gift from Dr. Golenbock; Addgene plasmid #13022) (0.5 and/or 0.2μg). ..

Article Title: ACE2-containing defensosomes serve as decoys to inhibit SARS-CoV-2 infection
Article Snippet: A549 ACE2 cells were generated using lentiviral transduction of a human ACE2 cDNA expressing plasmid (backbone: pLV-EF1a-IRES-Puro (Addgene 85132) as previously described [ ]. .. TLR7+ A549 were generated by lentiviral transduction of pcDNA3-TLR7-YFP (a gift from Doug Golenbock (Addgene plasmid # 13022; http://n2t.net/addgene:13022 ; RRID:Addgene_13022)). ..

Transfection:

other:

Article Title: Selective TLR ligand stimulation enhances in vivo mosquito-borne flavivirus pathogenicity.
Article Snippet: Lentivirus packaging vectors, pMDLg/pRRE, pRSV-Rev, and pCMV-VSV-G, were obtained from Addgene.

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TLR7 expression is upregulated in cartilage tissues of OA-patients and LPS-stimulated chondrocytes. Chondrocytes were treated with different concentrations of LPS (0, 1, 5, and 10 μg/ml) for 6, 12, and 24 h and cell supernatants were collected after culturing for 48 h. A. TLR7 expression in cartilage tissues of OA patients was detected by RT-qPCR. *P<0.05 compared with healthy controls. B. The viability of chondrocytes after LPS treatment. C. mRNA expression of TLR7 in LPS-induced chondrocytes was detected by RT-qPCR. D. Expression of TLR7 in LPS-induced chondrocytes was detected by western blot assay. C28/I2 chondrocytes were treated with different concentrations of LPS for 12 h; *P<0.05 and **P<0.01 compared with 0 μg/ml LPS treatment; #P<0.05 compared with 5 μg/ml LPS treatment. Columns present the mean ± SEM (n≥3).

Journal: American Journal of Translational Research

Article Title: Silencing of TLR7 protects against lipopolysaccharide-induced chondrocyte apoptosis and injury by blocking the p21-mediated JAK2/STAT3 pathway

doi:

Figure Lengend Snippet: TLR7 expression is upregulated in cartilage tissues of OA-patients and LPS-stimulated chondrocytes. Chondrocytes were treated with different concentrations of LPS (0, 1, 5, and 10 μg/ml) for 6, 12, and 24 h and cell supernatants were collected after culturing for 48 h. A. TLR7 expression in cartilage tissues of OA patients was detected by RT-qPCR. *P<0.05 compared with healthy controls. B. The viability of chondrocytes after LPS treatment. C. mRNA expression of TLR7 in LPS-induced chondrocytes was detected by RT-qPCR. D. Expression of TLR7 in LPS-induced chondrocytes was detected by western blot assay. C28/I2 chondrocytes were treated with different concentrations of LPS for 12 h; *P<0.05 and **P<0.01 compared with 0 μg/ml LPS treatment; #P<0.05 compared with 5 μg/ml LPS treatment. Columns present the mean ± SEM (n≥3).

Article Snippet: Besides, cells were transfected with the TLR7 overexpression plasmid (Addgene plasmid #13022) according to the method of Chamberlain et al. [ 28 ].

Techniques: Expressing, Quantitative RT-PCR, Western Blot

TLR7 silencing inhibited LPS-induced cell apoptosis and ECM degradation. Chondrocytes were transfected with control siRNA (si-NC, 1 μg/ml) and TLR7 siRNA (si-TLR7, 1 μg/ml) for 48 h and then cells were treated with 5 μg/ml LPS for 12 h. A, B. TLR7 expression in chondrocytes analyzed by western blot assay. C. The viability of chondrocytes evaluated by MTT assay. D. Apoptosis rate detected by flow cytometry analysis. E. Expression of cle-caspase3, BAX and Bcl-2 assayed by western blot analysis. F. Expression of MMP-3, MMP-13 and aggrecan assayed by western blot analysis. GAPDH was used as an internal control. Columns present the mean ± SEM (n≥3); *P<0.05 versus control; #P<0.05 versus LPS group.

Journal: American Journal of Translational Research

Article Title: Silencing of TLR7 protects against lipopolysaccharide-induced chondrocyte apoptosis and injury by blocking the p21-mediated JAK2/STAT3 pathway

doi:

Figure Lengend Snippet: TLR7 silencing inhibited LPS-induced cell apoptosis and ECM degradation. Chondrocytes were transfected with control siRNA (si-NC, 1 μg/ml) and TLR7 siRNA (si-TLR7, 1 μg/ml) for 48 h and then cells were treated with 5 μg/ml LPS for 12 h. A, B. TLR7 expression in chondrocytes analyzed by western blot assay. C. The viability of chondrocytes evaluated by MTT assay. D. Apoptosis rate detected by flow cytometry analysis. E. Expression of cle-caspase3, BAX and Bcl-2 assayed by western blot analysis. F. Expression of MMP-3, MMP-13 and aggrecan assayed by western blot analysis. GAPDH was used as an internal control. Columns present the mean ± SEM (n≥3); *P<0.05 versus control; #P<0.05 versus LPS group.

Article Snippet: Besides, cells were transfected with the TLR7 overexpression plasmid (Addgene plasmid #13022) according to the method of Chamberlain et al. [ 28 ].

Techniques: Transfection, Control, Expressing, Western Blot, MTT Assay, Flow Cytometry

Production of inflammatory mediators and cytokines in LPS-stimulated chondrocytes were reduced by TLR7 knockdown. Chondrocytes were transfected with si-NC and si-TLR7 for 48 h and then cells were treated with 5 μg/ml LPS for 12 h. A. The content of NO was detected in cells. B. The protein level of iNOS in cells was analyzed by western blot assay. C. mRNA expressions of IL-1β, TNF-α, and IL-6 in chondrocytes were assessed by RT-qPCR. D-F. The contents of IL-1β, TNF-α, and IL-6 in chondrocytes were detected through ELISA assay. Columns present the mean ± SEM (n≥3); *P<0.05 versus control; #P<0.05 versus LPS group.

Journal: American Journal of Translational Research

Article Title: Silencing of TLR7 protects against lipopolysaccharide-induced chondrocyte apoptosis and injury by blocking the p21-mediated JAK2/STAT3 pathway

doi:

Figure Lengend Snippet: Production of inflammatory mediators and cytokines in LPS-stimulated chondrocytes were reduced by TLR7 knockdown. Chondrocytes were transfected with si-NC and si-TLR7 for 48 h and then cells were treated with 5 μg/ml LPS for 12 h. A. The content of NO was detected in cells. B. The protein level of iNOS in cells was analyzed by western blot assay. C. mRNA expressions of IL-1β, TNF-α, and IL-6 in chondrocytes were assessed by RT-qPCR. D-F. The contents of IL-1β, TNF-α, and IL-6 in chondrocytes were detected through ELISA assay. Columns present the mean ± SEM (n≥3); *P<0.05 versus control; #P<0.05 versus LPS group.

Article Snippet: Besides, cells were transfected with the TLR7 overexpression plasmid (Addgene plasmid #13022) according to the method of Chamberlain et al. [ 28 ].

Techniques: Knockdown, Transfection, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control

TLR7 modulated the JAK2-STAT3 pathway by regulating p21 expression. Chondrocytes were transfected with si-NC, si-TLR7, and p21 siRNA (si-p21). Samples were collected after transfection for 48 h. A. mRNA expression of p21 in cartilage tissues of OA patients detected by RT-qPCR. B. p21 expression in LPS-induced chondrocytes detected by western blot assay. C. Relation between TLR7 and p21 evaluated by Pearson analysis. D-G. Protein expressions of p21, p-JAK2/JAK2 and p-STAT3/STAT3 assayed by western blot. GAPDH was used as an internal control. *, stands for P<0.05. Columns present the mean ± SEM (n≥3).

Journal: American Journal of Translational Research

Article Title: Silencing of TLR7 protects against lipopolysaccharide-induced chondrocyte apoptosis and injury by blocking the p21-mediated JAK2/STAT3 pathway

doi:

Figure Lengend Snippet: TLR7 modulated the JAK2-STAT3 pathway by regulating p21 expression. Chondrocytes were transfected with si-NC, si-TLR7, and p21 siRNA (si-p21). Samples were collected after transfection for 48 h. A. mRNA expression of p21 in cartilage tissues of OA patients detected by RT-qPCR. B. p21 expression in LPS-induced chondrocytes detected by western blot assay. C. Relation between TLR7 and p21 evaluated by Pearson analysis. D-G. Protein expressions of p21, p-JAK2/JAK2 and p-STAT3/STAT3 assayed by western blot. GAPDH was used as an internal control. *, stands for P<0.05. Columns present the mean ± SEM (n≥3).

Article Snippet: Besides, cells were transfected with the TLR7 overexpression plasmid (Addgene plasmid #13022) according to the method of Chamberlain et al. [ 28 ].

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot, Control

TLR7 silencing protected against chondrocyte apoptosis and injury by suppressing the p21-mediated JAK2/STAT3 pathway. Chondrocytes were transfected with si-NC, si-TLR7 and si-p21 and pre-treated with 0.25 µmol l-1 colivelin for 2 h. Samples were collected after transfection for 48 h. A. The viability of chondrocytes evaluated by MTT assay. B. Apoptosis rate of chondrocytes detected by apoptosis assay. C, D. Expressions of cle-caspase3, BAX and Bcl-2 assayed by western blot. E, F. Expressions of MMP-3, MMP-13 and aggrecan assayed by western blot analysis. G. mRNA expressions of IL-1β, TNF-α, and IL-6 in chondrocytes assessed by RT-qPCR. H-J. The levels of IL-1β, TNF-α, and IL-6 in chondrocytes were detected by ELISA. GAPDH was used as an internal control. Columns present the mean ± SEM (n≥3); *P<0.05 versus control; #P<0.05 versus LPS; $P<0.05 versus LPS+siTLR7.

Journal: American Journal of Translational Research

Article Title: Silencing of TLR7 protects against lipopolysaccharide-induced chondrocyte apoptosis and injury by blocking the p21-mediated JAK2/STAT3 pathway

doi:

Figure Lengend Snippet: TLR7 silencing protected against chondrocyte apoptosis and injury by suppressing the p21-mediated JAK2/STAT3 pathway. Chondrocytes were transfected with si-NC, si-TLR7 and si-p21 and pre-treated with 0.25 µmol l-1 colivelin for 2 h. Samples were collected after transfection for 48 h. A. The viability of chondrocytes evaluated by MTT assay. B. Apoptosis rate of chondrocytes detected by apoptosis assay. C, D. Expressions of cle-caspase3, BAX and Bcl-2 assayed by western blot. E, F. Expressions of MMP-3, MMP-13 and aggrecan assayed by western blot analysis. G. mRNA expressions of IL-1β, TNF-α, and IL-6 in chondrocytes assessed by RT-qPCR. H-J. The levels of IL-1β, TNF-α, and IL-6 in chondrocytes were detected by ELISA. GAPDH was used as an internal control. Columns present the mean ± SEM (n≥3); *P<0.05 versus control; #P<0.05 versus LPS; $P<0.05 versus LPS+siTLR7.

Article Snippet: Besides, cells were transfected with the TLR7 overexpression plasmid (Addgene plasmid #13022) according to the method of Chamberlain et al. [ 28 ].

Techniques: Transfection, MTT Assay, Apoptosis Assay, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control

The molecular mechanism of TLR7 regulating apoptosis, ECM degradation, and inflammation in the LPS-induced chondrocytes.

Journal: American Journal of Translational Research

Article Title: Silencing of TLR7 protects against lipopolysaccharide-induced chondrocyte apoptosis and injury by blocking the p21-mediated JAK2/STAT3 pathway

doi:

Figure Lengend Snippet: The molecular mechanism of TLR7 regulating apoptosis, ECM degradation, and inflammation in the LPS-induced chondrocytes.

Article Snippet: Besides, cells were transfected with the TLR7 overexpression plasmid (Addgene plasmid #13022) according to the method of Chamberlain et al. [ 28 ].

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